![]() ![]() Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4. 1992, Vol 12, Num 3, pp 185-192 ref : 28 ref CODEN JCIMDO ISSN 0271-9142 Scientific domain Immunology, immunopathology, allergology Publisher Kluwer/Plenum, New York, NY Publication country United States Document type Article Language English Keyword (fr) Diagnostic Etude comparative Evaluation performance Homme Hémopathie Immunodéficit acquis syndrome Immunopathologie Méthode immunoblotting Réaction chaîne polymérase Sérologie Technique ELISA Virus HIV1 Infection Lentivirinae Retroviridae Virose Virus immunodéficience humaine Virus Keyword (en) Diagnosis Comparative study Performance evaluation Human Hemopathy AIDS Immunopathology Immunoblotting assay Polymerase chain reaction Serology ELISA assay HIV-1 virus Infection Lentivirinae Retroviridae Viral disease Human immunodeficiency virus Virus Keyword (es) Diagnóstico Estudio comparativo Evaluación prestación Hombre Hemopatía Inmunodeficiencia adquirida síndrome Inmunopatología Reacción cadena polimerasa Serología Técnica ELISA HIV-1 virus Infección Lentivirinae Retroviridae Virosis Human immunodeficiency virus Virus Classification Pascal 002 Biological and medical sciences / 002B Medical sciences / 002B06 Immunopathology / 002B06E Immunological methods for diagnosis and exploration / 002B06E02 Serological tests Discipline Immunopathology Origin Inist-CNRS Database PASCAL INIST identifier 5360874 Frequency, Causes, and New Challenges of Indeterminate Results in Western Blot Confirmatory Testing for Antibodies to Human Immunodeficiency Virus - PMC Journal List Clin Vaccine Immunol v. molecular retrovirology sect., Bethesda MD 20892, United States Source A VASUDEVACHARI, M PSALLIDOPOULOS, M THOMPSON, L. Western blot indeterminate results in infected individuals are related to several factors such as early seroconversion, incomplete HIV-1 antibody evolution. Results support the recommendation that HIV-2 Western blot be included in the diagnostic algorithm for HIV-1/2 to followup negative or indeterminate HIV-1 Western blot results.CopyPermanent link Copy Indeterminate Western blot patterns in a cohort of individuals at high risk for human immunodeficiency virus (HIV-1) exposure Author DAVEY, R. HIV-1 seroconversion was observed in all 10 remaining samples during serological followup.Ĭonclusions: Two new HIV-2 seropositive cases were diagnosed using DAVIH–VIH-2 and HIV-2 Western blot in indeterminate HIV-1 Western blot samples. The Western blot assay is the gold standard for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). The two HIV-2–positive samples showed well defined reactivity to gp160, p53, p55 and p34 of HIV-1. All 12 ELISA-reactive samples belonged to the HIV-1 Western blot indeterminate group. Positive samples reacted against the p16, p26, gp36, p53, p56, p68 and gp105 proteins. ![]() Western blot analysis of the 12 ELISA-reactive samples confirmed two positive samples (16.67%), 4 negative (33.33%) and 6 indeterminate (50%). Results: Among all sera studied, HIV-2 ELISA identified 12 reactive serum samples (0.70%) and 1711 non-reactive (99.30%). ![]() The epidemiological interview established by Cuba's National Program for Prevention and Control Sexually-Transmitted Diseases and HIV/AIDS was applied to HIV-2 Western blot-positive patients. Western Blot INDETERMINATE was always accompanied with detectable RNA of HIV-1 with cases of true infection. Duplicate sera reactive by ELISA were confirmed by HIV-2 Western blot, results interpreted according to WHO criteria. In addition, this laboratory stage of having. Methods: HIV-2 reactivity was studied using the ELISA DAVIH–VIH-2 diagnostic kit (Cuba) in 1723 serum samples with negative or indeterminate results for HIV-1 Western blot from January 2005 through December 2008. Objetive: Investigate the contribution of HIV-2 antibodies to negative or indeterminate HIV-1 Western blot results in serum samples from 2005 through 2008 in Cuba. Indeterminate results constitute the main limitation of this test and HIV-2 infection is among their possible causes hence the importance of second-stage screening and confirmatory tests for HIV-2 infection. In Cuba, positive results in mixed HIV-1/2 screening assays are confirmed by HIV-1 Western blot. Diagnosis, laboratory techniques and procedures, antibodies, HIV-2, Western blot, enzyme-linked immunosorbent assay, algorithm, Cuba.ĪBSTRACT Introduction: Differentiating between HIV-1 and HIV-2 infection is the first step to understanding HIV transmission, epidemiology and pathogenesis in geographical areas where both viruses circulate. ![]()
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